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Home › Solutions › Cell Culture & Cryopreservation

Cell Culture & Cryopreservation

Cell Culture & Cryopreservation

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Cell Culture & Cryopreservation

Cells are the starting point of CGT and biopharmaceutical processes. From culture media and sera, buffers and water-for-injection-grade water to the preservation media used to freeze, thaw and move cells, we supply from one place the consumables that determine cell viability and reproducibility.

Product Lines
  • Cell culture media · sera · buffers
  • WFI-grade cell culture water
  • Cryopreservation media (CryoStor)
  • Hypothermic storage and shipping media (HypoThermosol)
  • Closed-system cryovials · automated thawing

Brands at This Stage

Corning Media · Buffer · FBS
Corning Media · Buffer · FBS
cGMP cell culture media · sera · reagents
Cell Culture & Cryopreservation
BioLife Solutions
BioLife Solutions
Biopreservation media chosen by commercially approved cell therapies
Cell Culture & Cryopreservation
BioLamina
BioLamina
Full-length human recombinant laminin substrates for stem cell culture
Cell Culture & Cryopreservation

Product Selection Information

These are the criteria to look at when choosing a product for your application, and the information that is helpful to share when you contact us. Specifications and options list only what has been confirmed.

BioLife Solutions · Cell Culture & Cryopreservation

Cryopreservation Solutions (CryoStor)

Ask About Cryopreservation Solution Selection
Main Uses
  • Cell cryopreservation (-70 ~ -196°C)
  • Biopreservation media for cells and tissues
Selection Criteria
  • DMSO concentration: a level suited to the cell type and route of administration
  • Pre-formulated with DMSO, or a DMSO-free base
  • Container format and volume: filling method · whether the process is closed
  • Freezing and thawing conditions and storage temperature
  • Technical documents to request: certificates of analysis · regulatory reference documents
Confirmed Specifications & Options
  • Pre-formulated with DMSO: CS2 · CS5 · CS10 (DMSO 2 · 5 · 10%)
  • CryoStor® CSB: DMSO-free base
  • Containers: 10 · 16 mL vials, 100 mL bottles
  • Containers: 100 · 1000 mL bags, 10 mL syringes
  • Serum-free, protein-free formulation; cGMP manufactured
  • FDA Master File held (cross-reference available)
What to Tell Us
  • Cell type and cell concentration at freezing
  • Desired DMSO concentration
  • Container format and volume needed per use
  • Cryopreservation solution and freezing method currently in use
  • Development stage and the quality and regulatory documents you need
Guide · Criteria for Selecting a Cell Cryopreservation Solution: What to Check in Cell Therapy Manufacturing
Biolaminin 521
BioLamina · Cell Culture & Cryopreservation

Biolaminin 521

Ask About Biolaminin 521
Main Uses
  • Culture of human pluripotent stem cells (hESC · hiPSC)
  • Maintenance of differentiated cells
  • Clinical cell applications (CTG grade)
Selection Criteria
  • Stage of use: research or clinical application
  • Grade: choose from CTG · MX · LN
  • Cell type and culture method
  • Level of requirements regarding animal-derived components
  • Technical documents to request: certificates of analysis · grade-specific quality documents
Confirmed Specifications & Options
  • Biolaminin 521 CTG: for clinical cell applications, Cell Therapy Grade
  • Biolaminin 521 MX: for research aimed at clinical application
  • Biolaminin 521 LN: for pluripotent stem cell culture
  • Type: full-length human recombinant laminin
  • CTG: free of animal-derived components (animal origin-free)
What to Tell Us
  • Cell type and purpose of culture
  • Development stage: research · preclinical · clinical
  • Desired grade (CTG · MX · LN)
  • Coating substrate currently in use
  • Culture scale and quantity needed
Corning Media · Buffer · FBS

Corning Media · Buffer · FBS

cGMP cell culture media · sera · reagents

Brand DetailsContact
Media
  • Classical Media — DMEM · DMEM/F12 · RPMI 1640 · MEM · IMDM · McCoy's 5A · Medium 199 and others
  • Serum-Free and Specialty Media (including Lymphocyte Serum-free Medium for cell therapy)
  • Corning® Powdered Media · Custom Media
Sera · Buffers · Water
  • Animal Sera (FBS and others)
  • Buffered Salt Solutions — DPBS · HBSS · TBS
  • Corning® Cell Culture Grade Water (tested to USP Water for Injection specifications)
Reagents · Supplements
  • Cell Culture Reagents · Supplements · Antibiotics
  • Growth Factors and Cytokines
BioLife Solutions

BioLife Solutions

Biopreservation media chosen by commercially approved cell therapies

Brand DetailsContact
Biopreservation Media
  • CryoStor® CS2 · CS5 · CS10 (pre-formulated with DMSO) · CryoStor® CSB (DMSO-free base)
  • HypoThermosol® FRS — hypothermic storage and shipping at 2–8°C
  • BloodStor® · Cell Thawing Media
Cell Processing Tools
  • CellSeal® Connect closed-system vials · CellSeal® CryoCase
  • ThawSTAR® automated thawing systems (vials · cryobags)
  • Signata CT-5® aseptic dispensing system
hPL · Cytokines
  • Human Platelet Lysate — Stemulate®-CP · nLiven PR® · T-Liven PR®
  • Recombinant cytokines IL-2 (animal origin-free) · IL-7 · IL-15 · FGF-2
BioLamina

BioLamina

Full-length human recombinant laminin substrates for stem cell culture

Brand DetailsContact
Biolaminin 521
  • Biolaminin 521 CTG — Cell Therapy Grade for clinical cell applications
  • Biolaminin 521 MX — for research aimed at clinical application
  • Biolaminin 521 LN — for pluripotent stem cell culture
Biolaminin isoforms
  • Biolaminin 511 · 421 · 411 LN
  • Biolaminin 332 · 221 · 211 · 121 · 111 LN
Biosilk (3D culture)
  • Biosilk
  • Biosilk 521

Guides for This Stage

All Guides
Cell Culture & Cryopreservation · Last reviewed 2026.10.09

Criteria for Selecting a Cell Cryopreservation Solution: What to Check in Cell Therapy Manufacturing

This guide sets out what to check when choosing a cryopreservation solution for cell therapy manufacturing, in this order: DMSO concentration and infusion reactions; animal-derived components; classification as ancillary material or excipient and qualification; freeze and thaw evaluation; and containers, stability and supply changes. All content is limited to what we confirmed directly in regulatory documents and peer-reviewed papers.

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Request a Quote · Cell Culture & Cryopreservation

Tell us your process stage and the product lines you need, and our technical specialists will respond quickly. TEL +82-2-6409-9338 · info@b2bio.co.kr

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